glass slide corning 75 × plain microscope slides  (Corning Life Sciences)

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Mechanical Compression of Coverslipped Tissue Sections During Heat-induced Antigen Retrieval Prevents Section Detachment and Preserves Tissue Morphology

doi: 10.1369/0022155419826940

Figure Lengend Snippet: Materials Used for the Pressurized Coverslipping and HIAR.

Article Snippet: Plain glass microscope slides (e.g., Corning frosted end, one side, 75 × 25 mm) , 3 , Ted Pella Inc.; 26005.

Techniques: Microscopy, Transferring, Staining

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Mechanical Compression of Coverslipped Tissue Sections During Heat-induced Antigen Retrieval Prevents Section Detachment and Preserves Tissue Morphology

doi: 10.1369/0022155419826940

Figure Lengend Snippet: Step-by-step instructions for pressure coverslipping of mounted tissue sections for heat-induced antigen retrieval treatment. The detailed protocol, (A) to (I), is given in the “Materials and Methods” section. Inset in (E), schematic illustration of a pressure-coverslipped slide: (1) microscope slide with mounted tissue section, (2) Teflon sheet, (3) second microscope slide, and (4) plastic tubing clamp.

Article Snippet: Plain glass microscope slides (e.g., Corning frosted end, one side, 75 × 25 mm) , 3 , Ted Pella Inc.; 26005.

Techniques: Microscopy

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Mechanical Compression of Coverslipped Tissue Sections During Heat-induced Antigen Retrieval Prevents Section Detachment and Preserves Tissue Morphology

doi: 10.1369/0022155419826940

Figure Lengend Snippet: Reattaching partially detached tissue sections using pressure coverslipping and HIAR. (A and A′) Three paraffin-embedded mouse kidney sections were mounted on a microscope slide. After paraffin removal and rehydration, two sections were still properly attached to the slide, whereas the third section was almost completely detached. (A′) shows the same slide as in (A) at a different angle. (B and B′) After HIAR treatment using pressure coverslipping, all three sections were properly attached to the slide. (B′) and (B) are at different angles. (C–E) The three sections from (A–B′) were immunolabeled with an antibody against the mineralocorticoid receptor (MR). In all three sections, specific nuclear labeling in distinct segments of the tubular epithelium that belong to the aldosterone-sensitive distal nephron was observed. In the reattached section (E and E′), artifactual DAB reaction product was observed at the margin of the section. Scale bars: (A–B′), 400 μm; (C–E′), 100 μm. Abbreviation: HIAR, heat-induced antigen retrieval.

Article Snippet: Plain glass microscope slides (e.g., Corning frosted end, one side, 75 × 25 mm) , 3 , Ted Pella Inc.; 26005.

Techniques: Microscopy, Immunolabeling, Labeling

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Mechanical Compression of Coverslipped Tissue Sections During Heat-induced Antigen Retrieval Prevents Section Detachment and Preserves Tissue Morphology

doi: 10.1369/0022155419826940

Figure Lengend Snippet: Pressure coverslipping during HIAR enables a distinctly more homogeneous immunolabeling of large tissue sections. (A–B″) A celloidin-embedded section of human tonsil tissue was mounted on a microscope slide (A). Overview (B) and higher magnifications (B′ and B″) of the same section after immunolabeling for PCNA and hematoxylin staining. Only a few weakly PCNA-labeled cells were observed in the lymphoid follicles. (C–D″) After HIAR without pressure coverslipping, disseminated focal detachment of the tissue section occurred—arrowheads and arrows in (D). These areas exhibited a more intense PCNA-immunolabeling and hematoxylin staining compared with adherent regions of the section—arrowhead in (D′), which resulted in nonhomogeneous labeling of the entire tissue section. (E–F″) Using HIAR with pressure coverslipping, the entire tissue section remained adherent to the slide (F). An intense and homogeneous immunolabeling for PCNA and hematoxylin staining was achieved (F′ and F″). Scale bars: (A, B, C, D, E, and F), 5 mm; (B′, B″, D′, D″, F′, and F″), 100 μm. Abbreviations: HIAR, heat-induced antigen retrieval; PCNA, proliferating cell nuclear antigen.

Article Snippet: Plain glass microscope slides (e.g., Corning frosted end, one side, 75 × 25 mm) , 3 , Ted Pella Inc.; 26005.

Techniques: Immunolabeling, Microscopy, Staining, Labeling

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Mechanical Compression of Coverslipped Tissue Sections During Heat-induced Antigen Retrieval Prevents Section Detachment and Preserves Tissue Morphology

doi: 10.1369/0022155419826940

Figure Lengend Snippet: Pressure coverslipping during HIAR preserves the delicate morphology of decalcified, celloidin-embedded human temporal bone (TB) sections. (A–B″) A celloidin-embedded human TB section after mounting on a microscope slide (A). Hematoxylin staining reveals the trabecular bony structure of the mastoid (left part of the section), cross-sections through the cavities of the middle and inner ear (middle part of the section), and the compact bone of the petrous apex (right part of the section). In particular, the delicate membranous structures of the cochlea—auditory portion of the inner ear (B′)—are vulnerable to the mechanical stress that occurs during histological processing and immunolabeling of the tissue. Without HIAR, no immunolabeling for the serum and glucocorticoid-regulated kinase 1 (SGK1) is observed in the organ of Corti (B″). (C–D′) After HIAR without pressure coverslipping, the tissue section almost completely detached from the slide (C and D). The delicate structures of the cochlea were almost completely disrupted (D′). The section could no longer be used for immunohistochemical labeling. (E–F″) After HIAR with pressure coverslipping, the section remained properly attached to the microscope slide (E). The morphology of all structures within the TB section was preserved (F), including the delicate architecture of the cochlea (F′). Immunolabeling for SGK1 showed nuclear and cytoplasmic signals in most supporting cells of the organ of Corti (F″). Scale bars: (A, B, C D, E, and F), 5 mm; (B′, D′, and F′), 1 mm; (B″ and F″), 100 μm. Abbreviation: HIAR, heat-induced antigen retrieval.

Article Snippet: Plain glass microscope slides (e.g., Corning frosted end, one side, 75 × 25 mm) , 3 , Ted Pella Inc.; 26005.

Techniques: Microscopy, Staining, Immunolabeling, Immunohistochemical staining, Labeling